根癌农杆菌与来自水稻成熟种子的愈伤组织共培养，将外源基因导入水稻愈伤组织，并获得了转基因植株．通过比较影响根癌农杆菌转化频率的各种因素，表明在转化过程中酚类化合物和单糖的加入使农杆菌的转化频率提高了０．９％～１７．４％．在共培养时农杆菌的稀释方式也是影响农杆菌转化频率的重要因素 Agrobacterium tumefaciens was co-cultured with callus from mature rice seeds, and the foreign genes were introduced into rice callus and transgenic plants were obtained. By comparing various factors affecting the transformation frequency of Agrobacterium tumefaciens, it was found that the transformation frequency of Agrobacterium increased by 0.9% ~ 17.4% during the transformation process by adding phenolic compounds and monosaccharides. Agar dilution during co-cultivation is also an important factor affecting the transformation frequency of Agrobacterium