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本文模拟卷柏复苏过程,检测在不同脱水和复水时间条件下卷柏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性、丙二醛(MDA)含量、膜透性及海藻糖含量,研究卷柏复苏过程中抗氧化系统响应机制及海藻糖变化规律,并探讨其复苏机理。研究结果表明:卷柏在含水量极低的情况下,通过复苏作用可基本恢复至初始状态;SOD、CAT和POD活性,MDA含量以及细胞膜透性均随着脱水时间的延长而上升,随着复水时间的延长而下降;海藻糖含量亦随着脱水时间的延长而增加,随着复水时间的延长而减少。卷柏抗氧化系统在复苏过程中启动其保护酶防御机制抵抗水分胁迫,而海藻糖在卷柏保护生物分子过程中发挥着重要作用。
This paper simulates the recovery process of Selaginella tamarisse and tests the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and malondialdehyde (MDA) under different dehydration and rehydration times ) Content, membrane permeability and trehalose content, the response mechanism of antioxidant system and trehalose in the process of recovery from the cypress were studied, and the recovery mechanism was discussed. The results showed that when the water content of Selaginella was very low, the initial recovery could be achieved by resuscitation; the activities of SOD, CAT and POD, the content of MDA and the permeability of cell membrane increased with the prolongation of dehydration time. The time of rehydration decreased and the content of trehalose increased with the extension of dehydration time and decreased with the rehydration time prolonged. The antioxidant system of Selaginelina starts its protective enzyme defense mechanism against the water stress in the process of resuscitation, and trehalose plays an important role in the protection of biomolecules by Selaginella.