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Objective. To investigate the clinical significance of glu- tathione- S- transferase GSTM1, GSTT1 and p53 codon 72 polymorphisms in cervical carcinogenesis. Methods. GST- M1, GSTT1 and p53 codon 72 polymorphisms together with human papillomavirus (HPV) types were examined in a total of 198 cervical smear samples using multiplex polymerase chain reaction (PCR) and PCR restriction fragment length polymorphism (RFLP) techniques. Results. Forty- two patients with high- grade squamous intraepithelial lesion (HSIL) had higher frequency of high- risk HPV and null GSTT1 genotype than 102 with low- grade SIL (LSIL) and 54 controls. Thirty- one patients with HSIL had also statistically higher frequency of null GSTT1 genotype than 28 with LSIL among 69 patients with high- risk HPV. There was no statistical difference in p53 Arg, Arg/Pro and Pro genotypes between SILs and controls with or without high- risk HPV. Conclusion. GSTT1 null genotype in cervical cell samples may be associated with more severe precancerous lesions of the cervix in a Japanese population. The p53 codon 72 polymorphism is unlikely to be related to HPV status and the onset of cervical cancer.
Objectives. To investigate the clinical significance of glu- tathione- S-transferase GSTM1, GSTT1 and p53 codon 72 polymorphisms in cervical carcinogenesis. Methods. GST- M1, GSTT1 and p53 codon 72 polymorphisms together with human papillomavirus (HPV) types were examined in a total of 198 cervical smear samples using a multiplex polymerase chain reaction (PCR) and PCR restriction fragment length polymorphism (RFLP) techniques. Results. Forty- two patients with high- grade squamous intraepithelial lesion (HSIL) had higher frequency of high- risk HPV and null GSTT1 genotype than 102 with low-grade SIL (LSIL) and 54 controls. Thirty-one patients with HSIL had also filavated higher frequency of null GSTT1 genotype than 28 with LSIL among 69 patients with high- risk HPV. There was no statistical difference in p53 Arg, Arg / Pro and Pro genotypes between SILs and controls with or without high- risk HPV. Conclusion. GSTT1 null genotype in cervical cell samples may be associated with more s evere precancerous lesions of the cervix in a Japanese population. The p53 codon 72 polymorphism is unlikely to be related to HPV status and the onset of cervical cancer.