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建立Real-time PCR鉴别羊奶粉的定性检测方法。通过对试剂盒提取DNA方法的改进得到适用于Realtime PCR测试的样品DNA。使用Real-time PCR法对羊奶粉中的羊源性成分进行鉴别。并通过普通PCR电泳方法对Real-time PCR方法进行验证,证明Real-time PCR方法的可行性。
The establishment of Real-time PCR differential detection of goat milk qualitative detection methods. The sample DNA suitable for the Realtime PCR test is obtained by the improvement of the method for extracting DNA from the kit. Real-time PCR was used to identify sheep-derived components in goat milk. Real-time PCR method was verified by ordinary PCR electrophoresis to prove the feasibility of Real-time PCR method.