建立普润注射液中 5 羟甲基糠醛 (5 HMF)的检测方法 ,采用D3 52 0 大孔吸附树脂柱分离葛根素与 5 HMF ,以紫外分光光度法于 2 84nm处测定洗脱液中 5 HMF的吸收度。结果表明 ,分离效果满意 ,可排除葛根素的干扰 ,测定方法平均回收率达 96 .9%,RSD为 0 .82 %(n =6 )。 To establish a method for the determination of 5-hydroxymethylfurfural (5 HMF) in Purin injection and the separation of Puerarin and 5 HMF by D3 52 0 macroporous resin column, and determine the content of 5 HMF in the eluate by UV spectrophotometry HMF absorption. The results showed that the separation was satisfactory and the interference of puerarin could be ruled out. The average recovery rate was 96.9% and the RSD was 0.82% (n = 6).