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芒(Miscanthus sinensis)具较高的生物学产量,是一种极具发展前景的纤维素类能源植物。以芒的8种不同基因型幼穗为外植体,进行了组织培养研究。结果表明,不同基因型芒在愈伤组织诱导率、胚性愈伤组织诱导率和胚性愈伤组织分化率等方面均存在显著差异。W89和W70均具较高的愈伤组织诱导率,分别为91.7%和89.1%;W69的外植体几乎全部褐化,且未能诱导出愈伤组织。W89、W70和W17的胚性愈伤组织分化率较高,达50%以上。另外,发现愈伤组织诱导率与细胞壁木质素含量间呈显著的负相关。该研究建立了稳定且有效的再生体系,并初步确定W89和W70可作为芒组织培养的理想材料,为芒的遗传转化、定向改良和良种快繁提供了技术支持。
Miscanthus sinensis has a high biological yield and is a promising cellulose-based energy plant. 8 different genotypes of young spikes as explants, tissue culture research. The results showed that there were significant differences in callus induction rate, embryogenic callus induction rate and embryogenic callus differentiation rate among different genotypes. Both W89 and W70 had higher callus induction rates of 91.7% and 89.1%, respectively. W69 explants were almost completely brown and could not induce callus. W89, W70 and W17 embryogenic callus higher differentiation rate of more than 50%. In addition, there was a significant negative correlation between callus induction and cell wall lignin content. The study established a stable and effective regeneration system and initially determined that W89 and W70 could be used as ideal materials for tissue culture, which provided technical support for genetic transformation, directional improvement and propagation of elite.