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目的研究麻黄连轺赤小豆汤三种组方(简称“三方”)对体外肝细胞损伤的保护作用及其机制。方法培养L-O2人肝细胞,采用H2O(2100μmol.L-1)体外诱导肝细胞损伤,检测细胞上清中天门冬氨酸转换酶(AST)和丙氨酸氨基转换酶(ALT)、丙二醛(MDA)的含量及超氧化物歧化酶(SOD)活力,cck-8法检测细胞活性。结果与阴性对照组比较,H2O(2100μmol.L-1)可抑制肝细胞活性,增加MDA的生成和ALT、AST的水平,降低SOD的活力(P<0.01,P<0.05);与模型组比较,麻黄连轺赤小豆汤三方均可拮抗H2O2对肝细胞的损伤作用,可促进细胞增殖,显著降低细胞上清ALT(除楸树皮方低剂量组)和AST水平(除楸树皮方高剂量组)及MDA含量(除梓白皮方低剂量组),明显提升SOD活力(P<0.01,P<0.05)。结论麻黄连轺赤小豆汤三方对体外肝细胞损伤均有直接保护作用,该作用可能与其抗氧化作用有关。
Objective To study the protective effect and mechanism of three groups (“Sifang”) of Ephedra Compound with Chixiaodou Decoction on hepatocellular injury in vitro. Methods L-O 2 human hepatocytes were cultured and induced by H2O2 (2100 μmol·L-1) in vitro. The aspartate aminotransferase (AST) and alanine aminotransferase (ALT) Dialdehyde (MDA) content and superoxide dismutase (SOD) activity, cck-8 assay cell activity. Results Compared with the negative control group, H2O (2100μmol.L-1) could inhibit the activity of hepatocytes, increase the production of MDA and the level of ALT and AST, and decrease the activity of SOD (P <0.01, P <0.05) , Ephedra Decoction Chih-Xia Dou Decoction can antagonize the H2O2 injury on the liver cells can promote cell proliferation, significantly reduce the cell supernatant ALT (except Catalpa Bark Fang low-dose group) and AST levels (except Catalpa Bark high dose Group) and MDA content (except Azadirachtin low dose group) significantly increased SOD activity (P <0.01, P <0.05). Conclusion Ephedra Decoction Chixiaodou Decoction has direct protective effect on hepatocellular injury in vitro, which may be related to its antioxidative effect.