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目的探讨湖州市水产品副溶血性弧菌(VP)污染与临床腹泻病例间的关联性。方法收集2014—2016年湖州市653份水产品和8 112例临床腹泻病例,分别对水产品与临床腹泻病例粪便或肛拭子标本进行定性VP检测、O群血清分群及tdh与trh毒力基因型检测。比较分析VP在水产品与临床腹泻病例中的流行分布特征。结果水产品的VP检出率为28.02%(183/653),其中淡水产品检出率为33.67%(132/392),海产品检出率为19.54%(51/261),差异有统计学意义(χ~2=14.823,P<0.001)。临床腹泻病例粪便或肛拭子标本的VP检出率为4.87%(395/8 112),其中暴露于水产动物及其制品的检出率达15.40%(170/1 104)。在时间分布上,水产品与粪便或肛拭子标本的检出率基本一致,均在7~9月检出率相对较高;在血清型分布上,二者均以O3、O4血清型为主;在毒力基因型分布上,临床腹泻病例以tdh(+)/trh(+)为主(60.51%,239/395),而水产品以tdh(-)/trh(-)为主(42.08%,77/183)。结论湖州市水产品副溶血性弧菌污染与临床病例感染在时间分布和生物学特征上具有一定关联,应进一步加强水产品中副溶血性弧菌的监测与管理。
Objective To investigate the association between the incidence of Vibrio parahaemolyticus (VP) in Huzhou and clinical cases of diarrhea. Methods A total of 653 aquatic products and 8 112 clinical diarrhea cases were collected from Huzhou City in 2014-2016. The fecal or anal swab specimens from aquatic products and clinical diarrhea were collected for qualitative VP detection. O group serogroups and tdh and trh virulence genes Type testing. Comparative analysis of VP in aquatic products and clinical cases of diarrhea in the epidemic distribution characteristics. Results The detection rate of VP in aquatic products was 28.02% (183/653), of which the detection rate of freshwater products was 33.67% (132/392) and the detection rate of seafood products was 19.54% (51/261), the difference was statistically significant Significance (χ ~ 2 = 14.823, P <0.001). The detection rate of VP in faecal or anal swab samples was 4.87% (395/8 112) in clinical diarrhea cases, of which the detection rate was 15.40% (170/1 104) when exposed to aquatic animals and their products. In terms of time distribution, the detection rates of aquatic products and fecal or anal swab specimens were basically the same, and the detection rates were relatively high from July to September. On the distribution of serotypes, the two types were both O3 and O4 serotypes In the distribution of virulence genotypes, tdh (+) / trh (+) was the predominant case of clinical diarrhea (60.51%, 239/395), while the aquatic products were dominated by tdh (-) / trh 42.08%, 77/183). Conclusions The contamination of Vibrio parahaemolyticus with aquatic products in Huzhou City has some correlation with the time distribution and biological characteristics of the infected cases. Therefore, the surveillance and management of Vibrio parahaemolyticus in aquatic products should be further strengthened.