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目的 :探讨喉癌患者外周血NK细胞 (NKC)杀伤活性及T淋巴细胞亚群的改变 ,以评估患者的免疫机能 ,预测预后。方法 :采用乳酸脱氢酶 (LDH)释放法 ,测定NKC杀伤活性 ;应用流式细胞仪检测T淋巴细胞亚群。结果 :喉癌组外周血NKC杀伤活性明显低于正常对照组及声带息肉组 (P <0 .0 5 ) ,病变程度越重 ,NKC杀伤活性越低。喉癌患者术后 3～ 6dNKC杀伤活性明显低于术前 (P <0 .0 5 ) ,7d后呈上升趋势。在NKC杀伤活性降低的同时 ,伴随CD3 、CD4及CD4/CD8比值下降 ,与正常对照组相比较 ,其差异有显著性意义 (P <0 .0 5 )。结论 :检测喉癌患者外周血NKC杀伤活性及T淋巴细胞亚群可客观反映机体免疫状态 ,具有重要临床意义
Objective: To investigate the changes of NK cell cytotoxicity and T lymphocyte subsets in peripheral blood of patients with laryngeal cancer so as to evaluate the immune function and prognosis of patients. Methods: The killing activity of NKC was determined by lactate dehydrogenase (LDH) release assay. The T lymphocyte subsets were detected by flow cytometry. Results: The NKC activity of peripheral blood in laryngeal carcinoma group was significantly lower than that in normal control group and vocal cord polyp group (P <0.05). The more severe the lesion was, the lower NKC activity was. The killing activity of NKC3 in laryngeal cancer patients after operation was significantly lower than that before operation (P <0.05), and was increased after 7 days. In addition to the decrease of NKC killing activity, accompanied with the decrease of CD3, CD4 and CD4 / CD8 ratio, the difference was significant compared with the normal control group (P <0.05). Conclusion: Detecting NKC activity in peripheral blood of patients with laryngocarcinoma and T lymphocyte subsets can objectively reflect the immune status of the patients, which has important clinical significance