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目的:研究Survivin基因在急性白血病(AL)患者中的表达及临床意义。方法:应用RT-PCR检测64例AL患者Survivin mRNA的表达水平,14例健康人为正常对照,K562、HL-60细胞株为阳性对照。结果:Survivin的阳性表达率高于正常对照,且在初治AL中的表达水平较正常对照组明显升高,治疗缓解后Survivin表达下降,复发时表达水平升高在初治AL患者中Survivin阳性患者缓解率低于表达阴性的患者。结论:Survivin基因表达的紊乱可能在AL的发病中起着重要作用,并且与AL复发和预后相关。
Objective: To investigate the expression of Survivin gene in patients with acute leukemia (AL) and its clinical significance. Methods: The expression of Survivin mRNA in 64 patients with AL was detected by RT-PCR. 14 healthy controls were normal controls, K562 and HL-60 cell lines were positive control. Results: The positive expression rate of Survivin was higher than that of normal control group, and the expression level of Survivin in primary AL was significantly higher than that in normal control group. Survivin expression was decreased after remission and the expression level of Survivin was higher in newly diagnosed AL patients Patients with remission rate lower than the negative expression of patients. CONCLUSIONS: The disturbance of Survivin gene expression may play an important role in the pathogenesis of AL, and is associated with AL recurrence and prognosis.