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采用选择性富集培养方法,从海洋沉积物中分离到能以高浓度蒽为唯一碳源且生长良好的优势菌,经形态学观察和生化检验初步鉴定此菌为黄杆菌属(Flavobacterium)。根据GC/MS方法测定了蒽的含量,经过5 d的优势菌培养对初始浓度为50 mg/L蒽的降解率可达77.6%。在萘、蒽和菲的混合物浓度分别为15 mg/L的情况下,该菌对菲的降解效率明显低于对蒽的降解效率,表现出对多环芳烃的酶降解具有很强的选择性。经对萃取中间代谢产物的质谱分析表明降解蒽的中间代谢产物主要有phthalic acid diisobutyl ester(邻苯二甲酸二异丁酯),9,10-anthracenedione(9,10-蒽醌)和dibutylphthalate(邻苯二甲酸二丁酯),说明它可能通过邻苯二甲酸途径来降解蒽。
The selective enrichment culture method was used to isolate the dominant bacteria that can grow anthracene as the sole carbon source from marine sediments. The bacteria were identified as Flavobacterium by morphological and biochemical tests. The anthracene content was determined by GC / MS method. After 5 days, the degradation rate of anthracene reached 77.6% for the initial concentration of 50 mg / L. The degradation efficiency of phenanthrene to phenanthrene was significantly lower than that of anthracene when the concentration of naphthalene, anthracene and phenanthrene was 15 mg / L, respectively, showing a strong selectivity for the degradation of polycyclic aromatic hydrocarbons . Mass spectrometric analysis of the intermediate metabolites showed that the intermediates metabolites of anthracene are phthalic acid diisobutyl ester, 9,10-anthracenedione and dibutylphthalate. Dibutyl phthalate), indicating that it may decompose anthracene through the phthalic acid pathway.