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目的:研究银杏叶提取物(EGb761)对黄曲霉素B1(AFB1)致大鼠肝细胞癌(HCC)发生发展的干预效果及其抗氧化作用。方法:实验大鼠按体质量随机分成3组:A组(AFB1组)、B组(AFB1+EGb761组)和C组(对照组)。分别于实验第14、28、42、55周进行肝活检并于第64周全部处死,观察大鼠肝脏癌前病变(γ-GT灶)、组织病理学及肝组织GSH-Px及MDA的变化。结果:在癌前期,第42及第55周时,B组单个γ-GT阳性灶的面积及灶总面积均显著小于相应时段A组的各项指标(P=0.000);动态观察定期肝脏活检的组织显示B组大鼠诱癌各时期肝脏损害均较A组轻,增生性病变发生亦较迟,HCC诱发率(26.92%)明显低于A组(76%)(P=0.000),对照组无肿瘤发生;B组GSH-Px活力较A组升高,而MDA含量较A组降低(P<0.05)。结论:EGb761能明显抑制AFB1诱发大鼠肝的癌前病变及延迟癌前病变向癌发展,这可能与其提高机体的抗氧化能力有关。
Objective: To study the intervention effect and anti-oxidative effect of ginkgo biloba extract (EGb761) on the development of hepatocellular carcinoma (HCC) induced by aflatoxin B1 (AFB1) in rats. Methods: The rats were randomly divided into three groups according to body weight: group A (AFB1), group B (AFB1 + EGb761) and group C (control). Liver biopsy was performed on the 14th, 28th, 42nd and 55th week of the experiment and sacrificed at the 64th week respectively. The changes of γ-GT lesion, histopathology, hepatic GSH-Px and MDA were observed . Results: At the precancerous period, the area and the total area of single γ-GT positive lesions in group B were significantly less than those in group A at the 42nd and 55th week (P = 0.000). The dynamic observation of regular liver biopsy Showed that the liver damage in group B was lighter than that in group A, and the proliferative lesions were also delayed. The rate of HCC induction (26.92%) was significantly lower than that in group A (76%) (P = 0.000) Group had no tumor. The activity of GSH-Px in group B was higher than that in group A, while the content of MDA in group B was lower than that in group A (P <0.05). Conclusion: EGb761 can significantly inhibit premalignant lesions of AFB1-induced liver and delay the development of precancerous lesions to cancer, which may be related to its ability to improve the body’s antioxidant capacity.