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目的探讨3种方法检测C-反应蛋白(CRP)对小儿长期发热的临床意义。方法采用免疫荧光干式定量法、散射比浊法、透射比浊法检测CRP的含量。结果实验组免疫荧光干式定量法检测CRP含量较对照组显著增高,差异具有统计学意义(P<0.05);散射比浊法及透射比浊法检测CRP含量较对照组均显著增高,差异均具有统计学意义(P<0.01)。与散射比浊法及透射比浊法比较,免疫荧光干式定量法检测CRP含量显著增高;而与散射比浊法比较,透射比浊法检测CRP含量显著降低。结论 3种检测方法中,免疫荧光干式定量法最简便,速度最快。散射比浊法最灵敏,准确度最高。透射比浊法较灵敏,准确度较免疫荧光干式定量法高,但较散射比浊法低。
Objective To investigate the clinical significance of three methods in detecting long-term fever in children with C-reactive protein (CRP). Methods Immunofluorescence quantitative method, turbidimetric method and turbidimetric method were used to detect the content of CRP. Results The level of CRP in the experimental group was significantly higher than that in the control group (P <0.05). The CRP levels in the experimental group were significantly higher than those in the control group by both the turbidimetric method and the turbidimetric method With statistical significance (P <0.01). Compared with the turbidimetric method and turbidimetric method, the content of CRP was significantly increased by immunofluorescence quantitative dry method. Compared with the turbidimetric method, the content of CRP was significantly decreased by turbidimetric method. Conclusion Among the three detection methods, the immunofluorescence dry-type quantitative method is the simplest and the fastest. Nephelometry most sensitive, the highest accuracy. Turbidimetric transmission method is more sensitive, accuracy than immunofluorescence dry quantitative high, but lower than the turbidimetric method.