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目的:研究螺旋藻复方对递增大负荷运动小鼠肝脏、心肌和骨骼肌的保护作用及对HSP70表达的影响。方法:用光学显微镜和电子显微镜分别观察递增大负荷运动和递增大负荷运动加螺旋藻复方对小鼠心肌、骨骼肌和肝组织的形态学影响。用免疫组化法(SP法)研究适量运动、递增大负荷运动、适量运动加螺旋藻复方和递增大负荷运动加螺旋藻复方对小鼠心肌细胞、骨骼肌细胞和肝细胞的HSP70蛋白表达的变化。结果:在光镜下,递增大负荷运动组小鼠心肌、骨骼肌和肝细胞出现了不同程度的水肿、变性和坏死,在电镜下,可见肌原纤维断裂、细胞肿胀、线粒体肿胀,空泡样变和细胞核核质溶解,可见凋亡小体。而递增大负荷运动加螺旋藻复方组小鼠心肌、骨骼肌和肝脏的病变程度明显减轻。运动各组小鼠心肌细胞、骨骼肌细胞和肝细胞HSP70表达均显著高于正常组(均P<0.01),适量运动加螺旋藻复方组小鼠心肌细胞、骨骼肌细胞和肝细胞HSP70表达均高于适量运动组(P<0.01或P<0.05)。递增大负荷运动加螺旋藻复方组小鼠心肌细胞、骨骼肌细胞和肝细胞HSP70表达均显著低于递增大负荷运动组(P<0.01或P<0.05),但与适量运动加螺旋藻复方组比较,无显著性差异(均P>0.05)。结论:螺旋藻复方能抵抗递增大负荷运动导致的组织细胞损伤,其分子机理可能与该方可提高运动诱导HSP70表达水平有关。
Objective: To study the protective effect of Spirulina on liver, myocardium and skeletal muscles and the effect on the expression of HSP70 in mice with increasing workload. METHODS: The morphological changes of myocardial, skeletal muscle and liver tissues in mice were observed with light microscope and electron microscope respectively. Immunohistochemistry (SP method) was used to study the expression of HSP70 protein in mouse cardiomyocytes, skeletal muscle cells, and hepatocytes after appropriate amount of exercise, incremental high-load exercise, moderate amount of exercise plus Spirulina compound, and incremental large-load exercise plus Spirulina compound. Variety. RESULTS: Under light microscopy, edema, degeneration, and necrosis of myocardium, skeletal muscle, and hepatocytes were observed in the progressively high-load exercise group. In electron microscopy, myofibril rupture, cell swelling, mitochondrial swelling, and vacuolization were observed. Changes and nuclear nucleoplasm dissolution, visible apoptotic bodies. However, the pathological changes of myocardium, skeletal muscle, and liver in mice with increasing SPP plus Spirulina compound group were significantly reduced. The expression of HSP70 in cardiomyocytes, skeletal muscle cells and hepatocytes of mice in each exercise group was significantly higher than that in the normal group (all P<0.01). The expression of HSP70 in mouse cardiomyocytes, skeletal muscle cells and hepatocytes in the appropriate amount of exercise plus spirulina compound group Higher than moderate exercise group (P<0.01 or P<0.05). The expression of HSP70 in cardiomyocytes, skeletal muscle cells, and hepatocytes in mice with increasing SHP combined with Spirulina group was significantly lower than that in the progressively high-load exercise group (P<0.01 or P<0.05), but it was associated with moderate exercise plus Spirulina compound group. There was no significant difference (all P>0.05). Conclusion: Spirulina compound can resist the injury of tissue cells caused by increasing heavy load exercise, and its molecular mechanism may be related to the increase of exercise-induced HSP70 expression level.