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目的评价升麻提取物SMT抗乙肝作用。方法采用乙型肝炎病毒(HBV)转基因小鼠模型,将小鼠分为模型对照组、阳性对照组、SMT1(75%乙醇提取物)组、SMT2(总皂苷)组及SMT3(总有机酸)组,各组分别给予不同的药物进行体内药效学实验。对各组HBV转基因小鼠血清中通过对人乙型肝炎E抗原(HBe Ag)、人乙型肝炎病毒表面抗原(HBs Ag)及乙肝病毒的脱氧核糖核酸(HBV-DNA)含量进行检测。结果对于小鼠血清中的HBs Ag含量,SMT1组和SMT3组均有所降低,且SMT1组与模型对照组比较差异有统计学意义(P<0.01);对于小鼠血清中的HBe Ag,各SMT组含量均有明显降低,与模型对照组比较差异有统计学意义(P<0.05);对于小鼠血清中的HBVDNA含量,SMT1组和SMT2组均有所降低,SMT1与模型对照组比较差异有统计学意义(P<0.05)。结论升麻提取物SMT1、SMT2、SMT3均具有明显抗HBV活性及肝保护作用,有待进一步提取、分离研究,对新药研发具有重要意义。
Objective To evaluate the anti-hepatitis B effect of Cimicifuga extract SMT. Methods The mice were divided into model control group, positive control group, SMT1 (75% ethanol extract) group, SMT2 (total saponin) group and SMT3 (total organic acid) Groups, each group were given different drugs for in vivo pharmacodynamics experiments. The serum of HBV transgenic mice in each group was detected by detecting the content of HBe Ag, HBs Ag and HBV DNA. Results The levels of HBsAg in serum of mice were decreased in SMT1 group and SMT3 group, and the difference between SMT1 group and model control group was statistically significant (P <0.01). For HBe Ag in mouse serum SMT group and SMT2 group were significantly decreased (P <0.05), and the content of HBVDNA in serum of SMT1 group and SMT2 group were decreased, the difference between SMT1 group and model control group was statistically significant There was statistical significance (P <0.05). Conclusion The anti-HBV activity and hepatic protective effect of the extracts of Cimicifuga sinensis SMT1, SMT2 and SMT3 are obvious and need to be further extracted and separated, which is of great significance for the research and development of new drugs.