Improvement of cardiac function and reversal of gap junction remodeling by Neuregulin-1β in volume-o

来源 :老年心脏病学杂志(英文版) | 被引量 : 0次 | 上传用户:ltiao9600
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
Objective We performed experiments using Neuregulin-1β (NRG-1β) treatment to determine a mechanism for the protective role derived from its beneficial effects by remodeling gap junctions (GJs) during heart failure (HF). Methods Rat models of HF were established by aortocaval fistula. Forty-eight rats were divided randomly into the HF (HF, n = 16), NRG-1β treatment (NRG, n = 16), and sham operation (S, n = 16) group. The rats in the NRG group were administered NRG-1β (10 μg/kg per day) for 7 days via the tail vein, whereas the other groups were injected with the same doses of saline. Twelve weeks after operation, Connexin 43 (Cx43) expression in single myocytes obtained from the left ventricle was determined by immunocytochemistry. Total protein was extracted from frozen left ventricular tissues for immunoblotting assay, and the ultrastructure of myocytes was observed by transmission electron microscopy. Results Compared with the HF group, the cardiac function of rats in the NRG group was markedly improved, irregular distribution and deceased Cx43 expression were relieved. The ultrastructure of myocytes was seriously damaged in HF rats, and NRG-1β reduced these pathological damages. Conclusions Short-term NRG-1β treatment can rescue pump failure in experimental models of volume overload-induced HF, which is related to the recovery of GJs structure and the improvement of Cx43 expression.
其他文献
目的 探讨富血小板血浆(PRP)对口腔黏膜微粒移植再造阴道创面愈合的促进作用.方法 自2011年3月至2011年9月,将7例先天性无阴道患者列入试验组,制作PRP均匀喷洒于再造阴道后的
目前在全世界范围内,冠心病已成为第一位的死亡原因.骨髓间充质干细胞(BMSCs)具有自我复制能力,可以向多种细胞分化,已有大量实验研究了它的分离、培养和体外诱导为心肌样细
理疗是利用人工或自然界物理因素作用于人体,使之产生有利的反应,达到预防和理疗疾病目的的方法,是各科室康复理疗的重要内容.理疗也是每一个住院患者根据疾病的不同需求进行
目的 评价吉非替尼与多西紫杉醇作为晚期非小细胞肺癌( NSCLC)二线治疗的临床疗效及安全性.方法 计算机检索PubMed、EMBASE、Cochrane图书馆、中国期刊全文数据库、中国生
目的:观察3种不同类型的脂肪乳剂[Intralipid (LCT)、Kalu (LCT/MCT)及Clin Oleic (LCT/MUFA)]在体外对人外周血中性粒细胞免疫功能的影响.方法:利用密度梯度法分离健康志愿
目的 探讨鼻内窥镜手术对慢性鼻窦炎的治疗效果,并对影响手术疗效的相关因素进行分析.方法 选择2006年6月至2009年4月收入的行鼻内窥镜的慢性鼻窦炎患者268例,观察慢性鼻窦炎
本文主要阐述了标准信息服务领域,如何更好地利用“大数据”来为标准服务。
以拟南芥ATHKl基因T-DNA插入所产生的缺失突变体和野生型ws(wassilewskija)生态型为材料,分析了它们在生理和基因表达方面的差异.结果表明突变体的离体叶片失水率明显大于野
前哨淋巴结状态可以比较准确地预测前列腺癌区域淋巴结的转移情况.近年来,国外关于运用SPECT/CT引导的前列腺前哨淋巴结检测做了大量研究,结果显示SPECT/CT引导的前列腺癌前
目的 通过多西紫杉醇处理前列腺癌细胞,探讨多西紫杉醇治疗雄激素抵抗前列腺癌可能的分子机制.方法 利用三种前列腺癌细胞系LNCaP、PC-3和CW22-rv1作为研究对象,用多西紫杉