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目的:研究辛伐他汀对HT29细胞的促凋亡作用,以及可能存在的调控机制。方法:将人结肠癌HT29细胞在不同浓度辛伐他汀的培养基中培养24、48、72 h,观察细胞的增殖情况,MTT法检测其抑制率;流式细胞术检测不同浓度辛伐他汀对HT29细胞凋亡率的影响,RT-PCR法观察HT29细胞Bcl-2、Bax mRNA水平,蛋白质印迹法观察HT29细胞Bcl-2、Bax蛋白表达情况。结果:不同浓度辛伐他汀对HT29细胞增殖有抑制作用,且其抑制作用同时存在浓度及时间效应(P<0.01);辛伐他汀对HT29细胞有促凋亡作用,抑制率随药物浓度增大而增加(P<0.05);PCR、蛋白质印迹法检测表明辛伐他汀可分别在基因及蛋白水平下调Bcl-2的表达及上调Bax的表达。结论:辛伐他汀可显著抑制HT29细胞的增殖,促进其凋亡,其可能与下调Bcl-2基因、上调Bax基因的表达有关。
Objective: To study the apoptosis-promoting effect of simvastatin on HT29 cells and the possible regulatory mechanisms. Methods: Human colon cancer HT29 cells were cultured in different concentrations of simvastatin for 24, 48 and 72 h. The proliferation of HT29 cells was observed by MTT assay. Flow cytometry was used to detect the effect of simvastatin on HT29 cell apoptosis rate. The Bcl-2 and Bax mRNA levels in HT29 cells were detected by RT-PCR. The protein expression of Bcl-2 and Bax in HT29 cells was detected by Western blotting. Results: Simvastatin at different concentrations could inhibit the proliferation of HT29 cells, and its inhibitory effect was both in concentration and time (P <0.01). Simvastatin had a pro-apoptotic effect on HT29 cells with increasing inhibition rate (P <0.05). PCR and Western blotting showed that simvastatin could down-regulate the expression of Bcl-2 and up-regulate the expression of Bax at the gene and protein level respectively. Conclusion: Simvastatin can significantly inhibit HT29 cell proliferation and promote apoptosis, which may be related to down-regulation of Bcl-2 gene and up-regulation of Bax gene expression.