目的：研究中老年男性血清睾酮和皮质醇含量变化及规律。方法：５８１例４０岁以上健康男性以每１０年为一年龄组，分为４组。以２０～３９岁健康男性１１０例作为对照组。均于上午８～１０时静脉采血，分离血清，应用放免分析方法检测血清睾酮和皮质醇含量。结果：（１）４０岁以上各组血清睾酮含量与年轻组比较明显下降（Ｐ＜００１），血清皮质醇含量逐渐增加，与对照组比较均差异显著（Ｐ＜００１）；（２）血清睾酮与皮质醇含量呈显著负相关（ｒ＝－０．３０９６，Ｐ＜００１）。结论：随增龄睾丸的内分泌功能逐渐下降，而肾上腺皮质功能则发生与之相反的变化 Objective: To study the changes and regularity of serum testosterone and cortisol in middle-aged and elderly men. Methods: 581 healthy males over the age of 40 were divided into 4 groups every 10 years. A total of 110 healthy males aged 20-39 years as control group. Blood samples were taken from 8 to 10 in the morning, serum was separated, and serum testosterone and cortisol levels were detected by radioimmunoassay. Results: (1) Serum testosterone levels were significantly decreased in all groups over 40 years old compared with young group (P <001), serum cortisol levels increased gradually, compared with the control group (P <001); ( 2) serum testosterone and cortisol content was significantly negatively correlated (r = -0.3096, P <0  01). CONCLUSIONS: The endocrine function gradually declines with increasing testis, whereas the adrenocortical function changes in the opposite direction