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近几年来,我国有不少单位开展了应用病毒防治松毛虫的研究,对于松毛虫质型多角体的染色方法,一般认为难于掌握,在显微镜下多角体不易识别。对此,我们做了一些研究和试验摸索。取得了较好的染色效果,现把几种方法介绍如下,仅供参考。一、澳酚兰染液染色法染液配方:HgCl_2 10克、95%酒精100毫升、溴酚兰100毫克。配法:先将Hgcl_2 10克溶于100毫升的
In recent years, many units in our country have carried out research on the application of the virus in the prevention and control of pine caterpillars. It is generally considered difficult to grasp the staining method of the polyhedron of the pine caterpillar type, and the polyhedron can not be easily identified under a microscope. In this regard, we have done some research and trial and error. Have achieved a better dyeing effect, are introduced below several methods, for reference purposes only. First, the United Arab phenol blue dye dyeing method dye formula: HgCl_2 10 grams, 95% alcohol 100 ml, bromophenol blue 100 mg. With law: first Hgcl_2 10 grams dissolved in 100 ml