论文部分内容阅读
探讨了不同胚龄鸡胚旋转不同时间后，脑细胞内游离Ｃａ２＋水平的变化。利用回转器模拟微重力生物效应及ｆｕｒａ－２比例荧光法，测定了孵化第６至１８ｄ鸡胚脑细胞游离Ｃａ２＋的浓度。结果显示：孵化第８至１７ｄ鸡胚经旋转不同时间后，其脑细胞内游离Ｃａ２＋浓度均有所下降，其中第１０ｄ鸡胚旋转４或７ｈ及第１３ｄ鸡胚旋转２４ｈ后，脑细胞内Ｃａ２＋浓度比对照组显著降低（Ｐ＜０．０１）。第１０ｄ和１３ｄ鸡胚分别旋转４ｈ和２４ｈ后再静置恢复相同时间，细胞内Ｃａ２＋浓度均有所增加，但仍低于对照组水平。结果表明：回转器旋转引起的鸡胚脑细胞内游离Ｃａ２＋水平降低是可逆的，而且在鸡胚脑后部的红色光灸，对鸡胚脑细胞内Ｃａ２＋水平降低的恢复具有一定促进作用
The changes of intracellular free Ca2 + levels in different time of embryo chicken embryos were investigated. The cyclotron was used to simulate the effects of microgravity and the fura-2 ratio fluorescence method was used to determine the concentration of free Ca2 + in chicken embryo brain cells from the 6th to the 18th day after hatching. The results showed that after 8 to 17 days of incubation, the free Ca2 + concentration in brain cells decreased at different time points. After 4 or 7 hours of embryo rotation and 24 hours of embryo rotation at 13 days, the intracellular Ca2 + The concentration was significantly lower than the control group (P <0.01). At the 10th and 13th day, the chick embryos were respectively rotated for 4h and 24h, and then resumed for the same time. The intracellular Ca2 + concentration was increased but still lower than that of the control group. The results showed that the decrease of intracellular free Ca2 + level in chicken embryo brain cells induced by rotator rotation was reversible, and the red light moxibustion in the posterior portion of chick embryo had a certain promotion effect on the reduction of intracellular Ca2 + level in chicken embryos