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以‘中农106’黄瓜为试材,对其进行不同温度和光照强度[正常温光25/15℃,光强(700±30)μmol·m-2·s-1;亚适宜温光18/12℃,光强(200±20)μmol·m-2·s-1;低温弱光12/8℃,光强75～100μmol·m-2·s-1]处理,研究亚适宜温光条件对结果期黄瓜生长、生理特性和氮(N)吸收的影响。结果表明,与正常温光处理相比,亚适宜温光和低温弱光处理下黄瓜株高、叶面积、果实大小与产量均明显下降,根系活力、叶绿素含量、果实品质和谷氨酰胺合成酶(GS)、谷氨酸合成酶(GOGAT)、谷氨酸脱氢酶(GDH)活性也随处理温度和光照强度降低而降低。亚适宜温光条件下,结果期黄瓜根系、叶片、果实中N含量均有所下降,亚适宜温光和低温弱光处理降低了结果期黄瓜N运转蛋白基因的表达,且温度和光照强度越低,黄瓜根系、叶片和果实中N运转蛋白基因表达量越低。说明亚适宜温光可能通过降低N运转蛋白基因的表达,抑制了N的吸收,降低N含量,进而抑制了黄瓜生长。
Using ’Zhongnong106’ cucumber as test material, different temperatures and light intensities [normal temperature 25/15 ℃, light intensity 700 ± 30 μmol · m-2 · s-1] 12 ℃, light intensity (200 ± 20) μmol · m-2 · s-1; low temperature and low light 12/8 ℃, light intensity 75 ~ 100μmol · m-2 · s-1] On cucumber growth, physiological characteristics and N (N) uptake during the period of fruiting. The results showed that the plant height, leaf area, fruit size and yield of cucumber were significantly decreased under suboptimal temperature, light and low temperature compared with normal temperature and light. Root activity, chlorophyll content, fruit quality and glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (GDH) activity also decreased with the decrease of treatment temperature and light intensity. Under moderate temperature and light conditions, the N content of cucumber roots, leaves and fruits decreased at the end of the study period, and the sub-suitable light and low temperature weak light treatments reduced the expression of N-working protein gene at the cucumber stage and the temperature and light intensity Low, the cucumber roots, leaves and fruits in the n-working protein gene expression is lower. These results indicated that suboptimal warming could inhibit the growth of cucumber by reducing the expression of N-working protein gene, inhibiting the absorption of N, decreasing the N content.