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目的： 在家蚕细胞和幼虫中表达日本血吸虫脂肪酸结合蛋白（ Ｓｊ１４） 基因。方法： 将该基因克隆于昆虫病毒转移载体ｐ Ｂａｃ Ｐ Ａ Ｋ Ｈｉｓ１ 中， 再与修饰的家蚕核型多角体病毒（ Ｂｍ Ｎ Ｐ Ｖ） 线性化 Ｄ Ｎ Ａ 共转染家蚕细胞，经体内重组得重组病毒 Ｂｍ Ｓｊ１４ ， 再将 Ｂｍ Ｓｊ１４ 感染家蚕细胞和幼虫以表达 Ｓｊ１４ ， 用 Ｗｅｓｔｅｒｎ ｂｌｏｔ 和间接 Ｅ Ｌ Ｉ Ｓ Ａ测定表达产物的抗原性。结果： Ｂｍ Ｓｊ１４ 感染家蚕细胞和幼虫后 Ｓｊ１４ 获得较高表达； 表达产物ｒ Ｓｊ１４ （ Ｈｉｓ） 为１８ｋ Ｄａ 的融合蛋白， 在家蚕细胞中的产量约为１００ μｇ／１ ×１０６ 细胞， 在培养上清中产量为３３ μｇ／ｍｌ； 在家蚕血淋巴中得量为４ ｍｇ／ｍｌ； 在家蚕幼虫组织的得量为４６ ｍｇ／ｇ 组织。 Ｗｅｓｔｅｒｎ ｂｌｏｔ 和间接 Ｅ Ｌ Ｉ Ｓ Ａ 显示ｒ Ｓｊ１４ （ Ｈｉｓ） 具有较高抗原性。结论： Ｓｊ１４ 在家蚕细胞和幼虫中获得高效表达且表达产物具有较高抗原性。
Objective: To express Schistosoma japonicum fatty acid binding protein (Sj14) gene in silkworm cells and larvae. Methods: The gene was cloned into the insect baculovirus transfer vector p Bac K A His1 and co-transfected with silkworm cells modified with Bm N P V linearized D N A. After in vivo recombination Recombinant virus Bm Sj14, and then Bm Sj14 infected silkworm cells and larvae to express Sj14, Western blot and indirect E L I S A determination of the expression of the antigenicity of the product. RESULTS: Sj14 was highly expressed in BmSj14 infected silkworm cells and larvae. The expressed product r Sj14 (His) was a 18 kDa fusion protein with a yield of about 100 μg / 1 × 106 in silkworm cells. Qingzhong yield of 33 μg / ml; in the hemolymph of silkworm, the amount of 4 mg / ml; in the silkworm larvae of the amount of 4 6 mg / g tissue. Western blot and indirect ELISAs show that rSj14 (His) is highly antigenic. Conclusion: Sj14 is highly expressed in silkworm cells and larvae and its expression product has high antigenicity.