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竹花叶病毒卫星RNA(satBaMV)是一个长度为836个核苷酸(不包括polyA)的单链正义RNA分子,可编码一20ku的卫星蛋白(P20).satBaMV的复制和包被需依赖竹花叶病毒(BaMV).P20是核酸结合蛋白,能促进satBaMV在寄主植物的长距离移动.利用细菌双杂交系统(BTH)和pull-downassays研究了P20自身、P20与BaMV蛋白以及BaMV蛋白之间的相互作用.研究表明:P20自身的相互作用是最强的;P20与甲基转移酶(MET)和衣壳蛋白(CP)之间有明显的相互作用;三基因连锁蛋白之间亦存在强的相互作用;CP与三基因连锁蛋白之间有明显的相互作用.删减分析表明,位于P20N端包括RNA结合位点在内的15个氨基酸是P20自身相互作用所必需的.N端缺失可导致P20间相互作用消失.P20的β折叠结构也是P20间相互作用所必需.此外,P20与烟草细胞色素C还原酶和β微管蛋白之间有较强的相互作用.BaMV蛋白与P20之间的同型和异型相互作用对BaMV及其卫星RNA在寄主植物中的移动起重要作用.
Bamboo mosaic virus satellite RNA (satBaMV) is a single-stranded sense RNA of 836 nucleotides in length (excluding polyA) that encodes a 20ku satellite protein (P20). Replication and coating of satBaMV is dependent on bamboo Mosaic virus (BaMV) .P20 is a nucleic acid binding protein that can promote the long-distance movement of satBaMV in host plants.We studied the relationship between P20 itself, BaMV protein and BaMV protein by using the bacterial two-hybrid system (BTH) and pull-downassays The interaction between P20 and methyltransferase (MET) and capsid protein (CP) showed obvious interaction. There was also a strong interaction between the three gene chain proteins The interaction between CP and the three-gene linkage protein showed that the 15 amino acids including the RNA binding site located at the P20 N-terminal were necessary for P20 self-interaction.The deletion of N-terminal Resulting in the disappearance of the interaction between P20.P20 β-sheet structure is also necessary for the interaction between P20 In addition, P20 and Tobacco cytochrome C reductase and β tubulin has a strong interaction between BaMV protein and P20 Homologous and heterotypic interactions of BaMV and Its satellite RNA plays an important role in the movement of host plants.