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一、香蕉外果皮 用成熟香蕉的外果皮观察导管,具有以下优点:材料易得,南北皆宜;不需用NaOH和NaHCO_3混合液解离,可直接压片观察;不需染色,用低倍镜就可清楚地观察到环(螺)纹导管。方法如下: 取成熟香蕉的外果皮,用小刀刮掉外果皮内侧疏松的部分,再用解剖针纵向分离,针间距2毫米左右。分离时可见有细丝相连。横向切取一小块组织,放在载玻片上,加一滴水,盖上盖玻片,用铅笔或解剖针钝端轻轻按压,用低倍镜观察,可清楚地看到环(螺)纹导管。为了加深对导管的理解,可将香蕉外果皮横向撕开,可见有
I. Exocarp of banana The exocarp observing the catheter with mature bananas has the following advantages: easy accessibility of materials; suitable for both north and south; no need to dissociate with NaOH and NaHCO_3 mixture, and can be directly observed by pressing; no staining is needed, with low magnification The mirror can clearly observe the ring (spiral) line catheter. The method is as follows: Take the exocarp of the mature banana, scrape off the loose part of the inner part of the outer skin with a knife, and then separate it longitudinally with an anatomical needle. The needle spacing is about 2 mm. Filaments are visible when separated. Cut a small piece of tissue laterally, place it on a glass slide, add a drop of water, cover it with a cover slip, and gently press with a blunt end of a pencil or an anatomical needle. Observe with a low power microscope to clearly see the ring (spiral) pattern. catheter. In order to deepen the understanding of the catheter, the banana peel can be torn horizontally.