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目的 将超抗原葡萄球菌肠毒素A(staphylococcalenterotoxinA ,SEA)分子转入肝癌细胞以增强其免疫原性 ,开辟肝癌免疫排斥的新途径。方法 从产SEA标准菌株中获得SEA基因全长片段 ,构建SEA逆转录真核表达载体 ,通过病毒包装和滴度测定 ,最后转导肝癌细胞 ,并进行T细胞杀伤实验 ,同时利用抗体阻断的方法研究递呈途径。结果 成功的获得了表达SEA人肝癌细胞株HHCSEA。结果显示微量表达的SEA蛋白即可引发高效的免疫活性。将细胞表面的HLA I分子利用抗体阻断后 ,杀伤活性明显降低。结论 肝癌细胞表达的SEA分子具有较高的免疫激活能力 ,且有可能主要通过HLA Ⅰ分子递呈。
Objective To transfer superantigenic staphylococcal enterotoxin A (SEA) molecules into hepatocellular carcinoma cells to enhance their immunogenicity and open up new ways for immune rejection of liver cancer. Methods The full-length SEA gene fragment was obtained from the SEA-producing standard strain. The SEA reverse transcription eukaryotic expression vector was constructed. Through the viral packaging and titer determination, the hepatoma cells were finally transduced, and the T cell killing experiment was performed. The antibodies were also blocked. Methods Study the presentation pathway. Results The human hepatoma cell line HHCSEA expressing SEA was successfully obtained. The results showed that a small amount of expressed SEA protein can lead to high-level immune activity. When the HLA I molecule on the cell surface was blocked with an antibody, the killing activity was significantly reduced. Conclusion The SEA molecules expressed in hepatocellular carcinoma cells have high immune activation ability and may be mainly presented by HLA I molecules.