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CSD(Cu/Zn superoxide dismutases)基因在拟南芥中参与多种胁迫应答,而miR398是CSD基因响应胁迫的原因。此处,我们成功克隆出了水稻的OsCSD2基因,并根据密码子的兼并性,突变了miR398作用的位点,但不改变其氨基酸序列,得到新的抗miR398的OsmCSD2,构建过表达载体并转入水稻中。对阳性转基因株系的分子鉴定表明,过表达植株的OsmCSD2基因获得了高表达。在重金属铜胁迫下,转基因株系表现出良好的抗逆性,H2O2含量显著低于野生型。
The CSD (Cu / Zn superoxide dismutases) gene is involved in multiple stress responses in Arabidopsis thaliana, and miR398 is responsible for the CSD gene response to stress. Here, we successfully cloned the OsCSD2 gene from rice and mutated the site of miR398 according to codon usage, but did not change its amino acid sequence to obtain a new OsmCSD2 against miR398. The overexpression vector was constructed and transfected Into the rice. The molecular identification of the positive transgenic lines showed that overexpression of OsmCSD2 gene was overexpressed. Under the heavy metal copper stress, the transgenic lines showed good resistance, H2O2 content was significantly lower than the wild type.