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本文应用PCR测序技术,对不同产地巴戟天(Morinda officinalis How)的rDNA ITS区片段进行检测,ITS全序列为567 bp,G/C含量为64.5%。本研究共获得17个单倍型,各单倍型呈高水平的树状演化,来自广东群体的单倍型显示是扩张的源头。分子变异等级分析(AMOVA)的计算结果显示,群体间变异占变异的比例(56.65%)大于群体内变异(43.35%),FST值为0.566 5,各群体间出现遗传分化。Mantel检验结果也显示基因流水平与地理距离呈正相关关系(R2=0.721 1),表明巴戟天群体间的亲缘关系与地理分布有较好的相关性。本研究为探讨巴戟天道地药材形成的分子机制以及谱系地理学的研究奠定基础。
In this paper, the rDNA ITS region of Morinda officinalis How from different areas was detected by PCR. The ITS sequence was 567 bp and the G / C content was 64.5%. A total of 17 haplotypes were obtained in this study. Each haplotype showed a high level of tree-like evolution. The haplotypes from Guangdong population showed the source of expansion. The results of molecular variance analysis (AMOVA) showed that the proportion of variation between groups (56.65%) was larger than that within the population (43.35%), and the FST value was 0.566 5. Genetic differentiation among the groups was observed. Mantel test also showed a positive correlation between gene flow and geographical distance (R2 = 0.721 1), indicating that the genetic relationship between Morinda officinalis populations and geographical distribution has a good correlation. This study laid the foundation for the study of the molecular mechanism of Morinda officinalis and the research of pedigree geography.