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目的 血管内皮细胞一氧化氮合成酶(endothelialnitricoxidesynthase,ecNOS)在体内催化一氧化氮合成。为研究一氧化氮合成酶基因转录的调控,对一氧化氮合成酶基因启动子序列进行功能分析。方法 以血管内皮细胞核提取物为材料,采用凝胶迁移实验和DNase足迹法实验。结果 启动子序列有3个区域与蛋白转录因子结合。其中(-106～-88)GC含量丰富,为转录因子SP1所结合;另外两个区域(-79～-64)和(-58～-44)覆盖文献报道中CTCF转录因子的结合区,但结合方式与CTCF不一致。结论 (-79～-64)和(-58～-44)区域可能结合有新的未曾报道的转录因子或存在SP1类似蛋白SP1多聚体的不完全特异性结合。
Objective Endothelial nitric oxide synthase (endothelial nitric oxide synthase, ecNOS) catalyzes the synthesis of nitric oxide in the body. In order to study the regulation of nitric oxide synthase gene transcription, the promoter sequence of nitric oxide synthase gene was analyzed. Methods Vascular endothelial cell nuclear extracts were used as materials, and gel migration assay and DNase footprinting assay were used. Results Three regions of the promoter sequence bound to the protein transcription factor. The (-106 ~ -88) GC content was abundant and was bound by the transcription factor SP1. The other two regions (-79 ~ -64) and (-58 ~ -44) covered the binding regions of CTCF transcription factors in the literature The combination is not the same as CTCF. Conclusions (-79 ~ -64) and (-58 ~ -44) regions may combine with incomplete or specific binding of new and unreported transcription factors or SP1 SP1-like protein SP1 multimers.