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目的探讨我国胃癌患者体细胞基因组水平ERBB2酪氨酸激酶编码区20、21外显子基因突变情况。方法随机选取2004年1月~2006年10月我院胃癌病理标本60例,正常对照25例,用套式PCR分别扩增ERBB2酪氨酸激酶编码区20、21外显子,PCR产物直接测序,并进行序列比对。结果55例胃癌组织、22例对照组织的ERBB2酪氨酸激酶编码区20、21外显子获得完整扩增;其中1例发现存在21外显子K874E错义突变,但反向测序未获证实,认定其为假阳性。结论我国胃癌患者ERBB2酪氨酸激酶编码区基因突变不是“频发的分子事件”。
Objective To investigate the mutations of exon 20 and 21 in the coding region of ERBB2 tyrosine kinase in somatic cell genome of Chinese patients with gastric cancer. Methods Sixty cases of gastric cancer in our hospital from January 2004 to October 2006 were randomly selected. Twenty-five normal controls were enrolled in this study. Exon 20 and 21 of ERBB2 tyrosine kinase coding region were amplified by nested PCR. The PCR products were directly sequenced , And the sequence alignment. Results The exon 20 and exon 21 of ERBB2 tyrosine kinase were completely amplified in 55 cases of gastric cancer and 22 cases of control tissues. One case was found to contain 21 exon K874E missense mutation, but reverse sequencing was not confirmed , Identified as false positive. Conclusion The mutation of ERBB2 tyrosine kinase coding region is not “frequent molecular event” in our country.