论文部分内容阅读
目的研究高砷饮水对儿童免疫功能的影响。方法于2006年选择某高砷饮水地区194名9～13岁小学生作为研究对象,采集家庭饮用水样和空腹晨尿,测定水砷和尿砷含量,按照水砷含量分为对照组(P<0.05mg/L)、暴露组(≥0.1mg/L),进行T淋巴细胞亚群、免疫球蛋白IgG、IgM、IgA和白细胞介素2(IL-2)测定,并对家庭一般情况、饮水水源情况、儿童健康状况、父母健康及职业暴露情况等进行问卷调查。结果对照组、暴露组儿童尿砷含量中位数分别为0.02和0.12mg/L,尿砷与水砷含量呈统计学相关。暴露组儿童T淋巴细胞(CD3+)、CD4+、CD8+亚群百分含量均值和CD4+/CD8+的均值低于对照组,经t检验,暴露组CD4+亚群百分含量和CD4+/CD8+显著低于对照组(P<0.05),而两组间T淋巴细胞(CD3+)、CD8+亚群百分含量差异均无统计学意义(P>0.05)。暴露组男生T淋巴细胞CD4+亚群百分含量显著低于对照组(P<0.05),暴露组女生CD4+亚群百分含量和CD4+/CD8+显著低于对照组(P<0.05)。暴露组儿童血清IgG含量高于对照组(P<0.05),血清IgM含量低于对照组(P<0.05),血清IgA含量两组间差异无统计学意义(P>0.05);对照组和暴露组男生血清免疫球蛋白含量分布情况与全体学生近似,女生血清免疫球蛋白含量差异无统计学意义。两组儿童白细胞介素2含量差异无统计学意义(P>0.05)。结论长期饮用砷含量在0.1mg/L以上的高砷水对儿童的免疫功能可能造成不利影响。儿童外周血T淋巴细胞亚群是水砷暴露非常敏感的健康效应指标之一。
Objective To study the effect of high arsenic drinking water on immune function in children. Methods A total of 194 primary school students from 9 to 13 years old in a high-arsenic drinking water district were selected as research objects in 2006. Household drinking water samples and fasting morning urine samples were collected for the determination of arsenic and arsenic in water samples. The arsenic contents were divided into control group (P < 0.05mg / L), exposed group (≥0.1mg / L), T lymphocyte subsets, immunoglobulin IgG, IgM, IgA and interleukin 2 (IL-2) Water conditions, children’s health status, parental health and occupational exposure, etc. Questionnaire survey. Results The median urinary arsenic levels of children in the control group and the exposed group were 0.02 and 0.12 mg / L, respectively. The urinary arsenic and water arsenic contents were statistically significant. The mean percentage of CD4 +, CD8 + subsets and CD4 + / CD8 + in exposed group were lower than those in control group. The percentages of CD4 + subsets and CD4 + / CD8 + in exposed group were significantly lower than those in control group (P <0.05). There was no significant difference in the percentages of T lymphocyte (CD3 +) and CD8 + subsets between the two groups (P> 0.05). The percentages of CD4 + subsets of T lymphocytes in exposed group were significantly lower than those in control group (P <0.05). The percentage of CD4 + subsets and CD4 + / CD8 + in exposed group were significantly lower than those in control group (P <0.05). The level of serum IgM in exposed group was higher than that in control group (P <0.05), and the level of IgM in serum was lower than that of control group (P <0.05). There was no significant difference in serum IgA level between the two groups (P> 0.05) The distribution of serum immunoglobulin in group boys was similar to that of all the students, but there was no significant difference in the serum immunoglobulin levels among girls. There was no significant difference in the content of interleukin 2 between the two groups (P> 0.05). Conclusion Long-term consumption of high-arsenic water with arsenic levels above 0.1mg / L may have adverse effects on children’s immune function. Children’s peripheral blood T lymphocyte subsets is one of the indicators of the health effects of water-arsenic exposure.