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切除 2 0～ 2 4月龄 C5 7BL /6NIA小鼠双侧卵巢 ,随后于颈部皮下植入可持续释放 60 d的含 1.7mgβ-雌二醇 (E2 )或含等剂量胆固醇的安慰剂。用免疫组织化学结合无偏性体视学方法 ,分析小鼠齿状回和海马 CA1区小胶质细胞和星形胶质细胞形态和数量的变化。结果发现 :60 d E2处理老年雌性小鼠不仅可以抑制神经胶质细胞的激活 ,而且可以明显降低小胶质细胞和星形胶质细胞的总数 ;神经胶质细胞在脑内免疫和炎症反应过程中起着非常重要的作用。本实验的结果提示 ,雌激素可能通过调节胶质细胞介导的脑内炎症反应通路 ,发挥其神经保护作用。
Bilateral ovaries from C57BL / 6NIA mice at 2 0 to 24 months of age were excised and subsequently subcutaneously transplanted into the neck subcutaneously with a placebo containing 1.7 mg of β-estradiol (E2) or an equivalent dose of cholesterol for 60 days. Immunohistochemistry and unbiased stereological methods were used to analyze the changes of morphology and number of microglia and astrocytes in the dentate gyrus and hippocampal CA1 area of mice. The results showed that 60 d E2 treatment not only inhibited the activation of glial cells, but also significantly reduced the total number of microglia and astrocytes; glial cells in the brain immune and inflammatory reaction process Plays a very important role. The results of this experiment suggest that estrogen may exert its neuroprotective effect by regulating glial cell-mediated inflammatory response pathways in the brain.