论文部分内容阅读
目的:该研究旨在基因水平上探讨补肾中药金匮肾气丸、右归丸对“劳倦过度、房事不节”肾阳虚小鼠模型“以药反证”影响的药物作用机理。方法:对照组、造模组、金匮肾气丸组和右归丸组,各组雄性小鼠分别为10只、15只、10只和10只。4组均予正常喂养,对照组和造模组每天灌胃蒸馏水0.5mL/只,两治疗组每天分别灌胃金匮肾气丸混悬液0.5mL/只和右归丸混悬液0.5mL/只,造模组和两治疗组均采用雄雌鼠比例1∶6同笼喂养和雄鼠每日游泳30～40min达4周,以诱导产生“劳倦过度、房事不节”肾阳虚证。用36K mouse genome array鼠脑芯片检测正常对照组和肾阳虚模型组鼠脑基因,并以二者荧光信号相对强度比值≥2和≤0.5筛选差异显著基因,并进一步用qRT-PCR对部分差异表达基因进行验证。结果:造模组诱导成功“劳倦过度、房事不节”肾阳虚鼠模型,造模组/对照组上调基因而两治疗组/造模组基因下调的有23个基因,其主要是与炎症/免疫、神经传递/信号转导等相关基因;造模组/对照组下调基因而治疗组/造模组基因上调的基因有6个,其主要是与细胞周期/细胞结构、神经传递/信号转导、转录等有关基因。qRT-PCR也证实了金匮肾气丸组/造模组、右归丸组/造模组和造模组/对照组的GH和Scgb3a1等6个差异基因表达。结论:金匮肾气丸、右归丸可使肾阳虚小鼠模型显著下调的激素、黑色素浓集素显著上调,并促进细胞增殖,从而在基因水平上探讨了金匮肾气丸和右归丸的药物作用机理。
OBJECTIVE: This study aimed to investigate the mechanism of action of Bushen Chinese Herbs Jinkui Shenqi Pills and Yougui Pills on the effects of kidney-busting Chinese medicines Jinkui Shenqi Pills and Yougui Pills on the effects of overwork fatigue, intercourse failure, and “kidney yang deficiency” mice model “drug reversal syndrome”. . Methods: Control group, model group, Jinkuishenqi Pill group and Yougui Pill group. The number of male mice in each group was 10, 15, 10 and 10 respectively. The four groups were all fed normally. The control group and the model group were given distilled water (0.5mL per day). The two treatment groups were intragastrically administered with 0.5mL of Jinkuishenqi Pill suspension per day and 0.5mL of Yougui pill suspension. Only in the modeling group and the two treatment groups male and female rats were fed 1:6 in the same cage and the males swimming for 30-40 minutes per day for 4 weeks to induce “overwork fatigue, intercourse failure” and kidney yang deficiency. certificate. The brain genes of the normal control group and the kidney-yang-deficiency model group were detected with a 36K mouse genome array brain microarray. The differentially expressed genes were screened by the relative intensity ratios of the fluorescence signals ≥ 2 and ≤ 0.5, and further partial differences were identified by qRT-PCR. Express genes for verification. RESULTS: The model group induced successful “overwork and intercourse failure ” kidney-yang deficiency mouse model, the modeling group/control group up-regulated genes, and the two treatment groups/modeling group genes had 23 genes down-regulated, mainly Genes related to inflammation/immunity, neurotransmission/signal transduction, etc. Genes in the model/control group were down-regulated while 6 genes were up-regulated in the treatment/modeling group, mainly related to cell cycle/cell structure and neurotransmission. / Signal transduction, transcription and other related genes. qRT-PCR also confirmed the expression of six differential genes such as GH and Scgb3a1 in the Jinkuishenqi Pill/modeling group, Yougui pill/modeling group, and the model/control group. Conclusion: Jinkuishenqi Pills and Yougui Pill can significantly upregulate the hormone and melanin concentration in the kidney-deficient mouse model, and promote cell proliferation. Therefore, Jinkui Shenqi Pill and right-guiding are discussed at the gene level. The mechanism of drug action of pills.