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目的:评价佐剂SWZY对弱免疫原性黑色素瘤瘤苗的免疫增强作用。方法:C57BL/6小鼠分为6组,实验组分别用5种不同配方的佐剂(FCA,FCA+IL-2+GM-CSF,FIA+IL-2+GM-CSF,FIA+SWZY,FIA+SWZY+IL-2+GM-CSF)和照射灭活的小鼠黑色素瘤细胞株D5制成瘤苗免疫小鼠,对照组免疫用不加任何佐剂的灭活D5黑色素瘤细胞。末次免疫后3d各组取半数动物检测DTH反应、脾细胞的杀伤活性以及免疫小鼠血清及脾细胞培养上清中IFN-γ和IL-10的水平,剩余半数动物接种未灭活的D5黑色素瘤细胞,3周后再次检测上述各免疫学参数。结果:与对照组小鼠比较,各实验组小鼠的DTH反应及脾细胞的杀伤活性均明显升高(P<0.05),但成瘤后随着肿瘤的增大,则呈下降趋势。成瘤前各实验组小鼠血清及脾细胞培养上清中IFN-γ的水平均高于对照组小鼠(P<0.05),但IL-10的水平均低于对照组小鼠。成瘤后各实验组及对照组小鼠血清及脾细胞培养上清中IFN-γ的水平均下降,而IL-10的水平均明显上升,其中FCA瘤苗组和FIA+SWZY瘤苗组免疫小鼠的血清及脾细胞培养上清中IFN-γ的水平仍高于对照组小鼠(P<0.05),IL-10的水平仍低于对照组小鼠(P<0.05)。结论:用5种佐剂配方制成的瘤苗免疫小鼠均能诱导对弱免疫原性肿瘤的细胞免疫应答,并增强Th1型细胞免疫的应答,但随着肿瘤的形成和逐渐进展,细胞免疫应答的效应逐渐减弱。其中佐剂SWZY与FCA的作用相当,但前者的毒副作用较小,有可能成为一种新型的人用肿瘤疫苗的佐剂。
Objective: To evaluate the immune enhancing effect of adjuvant SWZY on weak immunogenic melanoma tumor vaccine. Methods: C57BL / 6 mice were divided into 6 groups. The experimental groups were treated with 5 different formulations of adjuvant (FCA, FCA + IL-2 + GM-CSF, FIA + IL-2 + GM-CSF, FIA + SWZY, FIA + SWZY + IL-2 + GM-CSF) and irradiated inactivated mouse melanoma cell line D5 were used to immunize mice. The control group was immunized with inactivated D5 melanoma cells without any adjuvant. Three days after the last immunization, half of the animals in each group were tested for DTH response, cytotoxic activity of spleen cells, levels of IFN-γ and IL-10 in the serum and splenocyte culture supernatant of the immunized mice, and the remaining half animals were inoculated with inactivated D5 melanin Tumor cells, three weeks after re-testing the above immunological parameters. Results: Compared with the control group, the DTH response and the cytotoxic activity of spleen cells in each experimental group were significantly increased (P <0.05), but decreased with the increase of tumor size after tumor formation. The levels of IFN-γ in the culture supernatant of the mice in each experimental group before tumorigenesis were higher than those in the control group (P <0.05), but the levels of IL-10 in the experimental group were lower than those in the control group. The levels of IFN-γ in serum and spleen cell culture supernatant of mice in each experimental group and control group decreased after tumorigenesis, while the levels of IL-10 increased significantly. The immunized mice in FCA group and FIA + SWZY group The level of IFN-γ in the serum of the mice and the supernatant of the spleen cells were still higher than those in the control group (P <0.05), while the level of IL-10 in the supernatant was still lower than that of the control group (P <0.05). CONCLUSION: All the mice immunized with 5 kinds of adjuvant vaccine can induce cellular immune response to weakly immunogenic tumor and enhance the response of Th1-type cellular immunity. However, with the formation and gradual progress of the tumor, the cells The effect of the immune response diminishes. Adjuvant SWZY and FCA role of considerable, but the former has less side effects, it may become a new human tumor vaccine adjuvant.