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水凝胶微孔阵列是细胞培养的新型基板软材料,其微孔形貌对细胞的行为产生直接的影响.但传统水凝胶微孔阵列形貌的表征手段缺乏在水溶液中原位和可逆表征的能力.本文以水溶液中的氧气为还原电对,应用扫描电化学显微镜(SECM)对水溶液中的聚乙二醇二甲基丙烯酸酯水凝胶微孔阵列的形貌进行了原位表征,得到了水凝胶微孔阵列表面的二维孔径和三维形貌信息,开发出采用SECM对水凝胶微孔阵列形貌进行原位、可逆、无损表征及提供三维形貌信息的新方法.
Hydrogel microwell array is a new type of substrate soft material for cell culture, and the morphology of microwells has a direct effect on the behavior of cells, but the characterization of traditional hydrogel micropore array morphology lacks in situ and reversible characterization in aqueous solution The morphology of the micro-pore array of polyethylene glycol dimethacrylate hydrogel in aqueous solution was characterized by in situ scanning electron microscopy (SECM) The two-dimensional pore size and three-dimensional topography information of hydrogel microwell arrays were obtained. A new method was developed for the in situ, reversible and nondestructive characterization of hydrogel microwell arrays using SECM and to provide three-dimensional topography information.