本文提出邻氨基酚 (OAP)-H2O2-辣根过氧化物酶（HRP）伏安酶联免疫分析体系并用于人血清中甲胎蛋白(αFP)的测定。该方法是将HRP催化H2O 2 氧化 OAP 的酶催化反应与邻氨基酚的氧化中间产物(邻苯醌亚胺)在滴汞电极上的还原反应相偶合, 在BR缓冲溶液中, 在-0.87 V (vs.SCE) 左右产生灵敏的极谱波。根据测定标记在甲胎蛋白抗体上的HRP的量, 求得发生免疫反应的αFP的含量。该方法对甲胎蛋白测定的线性范围为1.25～400 mg/L。用所建立的方法对病人血清样品进行了测定, 并与酶联免疫吸附测定光度法(ELISA)进行对照,二者相关性很好。“,”A new voltammetric enzyme immunoassay with coupled rea ction for the determination of α-fetoprotein(αFP) in human serum is d eveloped. The method is based on coupling the oxidation reaction of OAP-H2O 2 catalyzed by αFPAb-HRP with the electro-reduction of the oxidizing prod uct of OAP to measure the activity of αFPAb-HRP, and the latter reaction e xhibits a sensitive second order derivative linear sweep voltammetric wave at -0 .87 V(SCE) in BR buffer, therefore, αFP could be measured. The sensitivity of present method to detect αFPAb-HRP and αFP is higher than enzyme -linked immunosorbent assay(ELISA). Some patient human serum samples have been analyzed with satisfactory results.