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应用UHPLC-HR-MSn技术分析检测苦碟子注射液中原型成分及其在大鼠体内的代谢物。尾静脉注射苦碟子注射液后,收集给药前后的大鼠血浆,经固相萃取进行处理后,采用ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)色谱柱和0.1%甲酸水溶液(A)-乙腈(B)流动相系统进行梯度洗脱。应用LTQ-Orbitrap质谱负离子模式检测苦碟子注射液中的化学成分以及血浆样品中的原型成分和代谢产物。根据精确相对分子质量数据和多级质谱碎片,结合对照品比对,从苦碟子注射液中分析鉴定了53个成分,包括18个有机酸、15个黄酮、18个倍半萜内酯、2个核苷;从大鼠血浆中分析检测了38个原型及代谢产物,主要代谢途径为甲基化、羟基化、水解、葡萄糖酸酸化及它们的复合反应等。应用UHPLC-LTQ-Orbitrap高分辨液质联用技术较为全面地阐明了苦碟子注射液化学成分及其在大鼠血浆中的移行成分,为进一步研究苦碟子注射液体内代谢过程以及药效物质基础提供了科学依据。
Application of UHPLC-HR-MSn technology in the determination of kudzu injection prototype components and their metabolites in rats. After tail vein injection of Kudiezi injection, the plasma of rats before and after administration was collected and subjected to solid-phase extraction. The samples were collected on a ACQUITY UPLC BEH C 18 (2.1 mm × 100 mm, 1.7 μm) column and 0.1% formic acid Aqueous solution (A) - acetonitrile (B) mobile phase system gradient elution. LTQ-Orbitrap mass spectrometry negative ion mode was used to test the chemical composition of Kudiezi injection as well as the prototype components and metabolites in plasma samples. According to the accurate relative molecular mass data and multi-stage MS fragments, compared with the control samples, 53 components including 18 organic acids, 15 flavones, 18 sesquiterpene lactones and 2 Nucleosides. 38 prototypes and metabolites were detected from the plasma of rats. The main metabolic pathways were methylation, hydroxylation, hydrolysis, gluconic acid acidification and their complex reactions. The application of UHPLC-LTQ-Orbitrap high-resolution liquid chromatography-mass spectrometry (LC-MS / MS) technique to elucidate the chemical constituents of Kudiezi injection and its migration components in rat plasma is more comprehensive. In order to further study the metabolic process and pharmacodynamic substance base of Kudiezi injection, Provide a scientific basis.