乳蛋白是研究真核细胞基因表达和调控的良好系统。mRNA是基因表达的重要环节。本文以乳腺为材料,从授乳豚鼠乳腺中分离得到能指导乳蛋白合成的Poly(A)~+RNA,在转录和翻译水平上研究蛋白质的合成。 材料与方法 按Glisin等所述的CsCl离心法,从分娩后3～6d的授乳豚鼠提取乳腺细胞RNA;按Aviv等所述的Oligo(dT)-Cellulose 亲和层析法,从总RNA中进行poly(A)~+RNA的纯化; 按 Milk protein is a good system for studying the gene expression and regulation of eukaryotic cells. mRNA is an important part of gene expression. In this paper, Poly (A) ~ + RNA, which can guide the synthesis of milk protein, was isolated from the mammary gland of mammary gland of mammary gland, and the synthesis of protein was studied at the transcriptional and translational levels. Materials and Methods Mammary gland cell RNA was extracted from celled guinea pigs 3 to 6 days after delivery by CsCl centrifugation as described by Glisin et al. The total cellular RNA was extracted from total RNA by Oligo (dT) -Cellulose affinity chromatography as described by Aviv et al Purification of poly (A) ~ + RNA; press