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制备了Nb2O5/石墨烯修饰玻碳电极（Nb2O5/RGO/GCE），建立了一种简便、灵敏检测绿原酸的电化学方法。用氧化石墨烯（GO）和五氯化铌（NbCl5）一步溶剂热法制备Nb2O5/RGO复合材料，并用扫描电子显微镜（SEM）对其进行形貌表征。采用循环伏安法（CV）和方波伏安法（SWV）研究了绿原酸在Nb2O5/RGO/GCE上的电化学行为。结果发现，Nb2O5/RGO复合材料能显著增强绿原酸的电化学活性。对实验条件（如pH值、扫描速率与富集时间等）进行了优化。在最佳条件下，绿原酸的氧化峰电流与浓度在5.0×10-7~1.2×10-5mol/L范围内呈良好的线性关系，检出限为2.0×10-7mol/L。采用修饰电极测定各种药物中绿原酸的含量，得到加标回收率为96.6%~101.5%。该方法具有良好的灵敏度和稳定性，已成功应用于药物中绿原酸含量的测定。
A Nb2O5 / graphene modified glassy carbon electrode (Nb2O5 / RGO / GCE) was prepared and a simple and sensitive electrochemical method for the determination of chlorogenic acid was established. Nb2O5 / RGO composites were prepared by one-step solvothermal method using graphene oxide (GO) and niobium pentachloride (NbCl5), and characterized by scanning electron microscopy (SEM). The electrochemical behavior of chlorogenic acid on Nb2O5 / RGO / GCE was investigated by cyclic voltammetry (CV) and square wave voltammetry (SWV). The results show that, Nb2O5 / RGO composite material can significantly enhance the electrochemical activity of chlorogenic acid. The experimental conditions (such as pH, scan rate and enrichment time) were optimized. Under the optimum conditions, the oxidation peak of chlorogenic acid showed a good linear relationship with the concentration of 5.0 × 10-7 ~ 1.2 × 10-5mol / L with the detection limit of 2.0 × 10-7mol / L. The modified electrode was used to determine the content of chlorogenic acid in various drugs, and the recoveries were 96.6% ~ 101.5%. The method has good sensitivity and stability and has been successfully applied to the determination of chlorogenic acid in medicines.