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目的：对鼻咽癌细胞株ＳＵＮＥ１异质性进行研究；方法：应用软琼脂集落形成实验、动物实验及ＲＮＡ指纹图谱差异显示对ＳＵＮＥ１４亚株５８Ｆ，９４Ｅ，６１０Ｂ，１３９Ｂ进行体外转化能力、成瘤性、转移能力及基因表达差异进行研究。结果：①４亚株体外转化能力、成瘤性和转移能力存在差异；②筛选出高成瘤、高转移潜能的亚株５８Ｆ、９４Ｅ，只成瘤不转移的６１０Ｂ，不成瘤的１３９Ｂ；③４亚株的基因表达存在异质性，并筛选出３条迁泳率不一致的差异片段。结论：ＳＵＮＥ１４亚株为起源于同一母系的不同的单克隆细胞亚株；为筛选和克隆ＮＰＣ发生发展相关基因的研究提供实验材料，亦为ＮＰＣ的基因治疗、免疫治疗及化学治疗的研究提供实验依据。
Objective: To investigate the heterogeneity of the nasopharyngeal carcinoma cell line SUNE1. Methods: The soft agar colony formation assay, animal experiments, and RNA fingerprinting showed that the sub-strains of SUNE14 were 58F, 94E, 6 10B, 13 9B studies in vitro transformation ability, tumorigenicity, metastasis ability and gene expression differences. RESULTS: There were differences in in vitro transformation ability, tumorigenicity, and metastasis capacity of 14 sub-strains; 2) Sub-strains 5-8F, 9-4E with high tumorigenicity and high metastatic potential were selected; 6-10Bs that did not metastasize only became unsuccessful. The gene expressions of tumors in the 13 9B; 34 substrains were heterogeneity, and 3 inconsistent differential fragments were selected. Conclusion: SUNE14 sub-strain is a sub-strain of different monoclonal cells originating from the same maternal line; it provides experimental materials for the screening and cloning of genes involved in the development of NPCs. It is also a study of gene therapy, immunotherapy and chemotherapy for NPCs. Provide experimental basis.