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目的 研究促吞噬肽 (tuftsin)和肿瘤坏死因子 (tumornecrosisfactor α,TNF α)的相互关系。方法 建立大鼠有脾和无脾动物模型 ,通过静脉注射TNF模拟病理状态下血中TNF浓度升高 ,利用反向高效液相色谱法检测血清中促吞噬肽的水平。结果 半脾切除组和全脾切除组术后 2周促吞噬肽水平下降 (P <0 .0 0 1) ,并以后者为著。注射TNF各组注射后d 3,d 5血清中促吞噬肽水平显著降低 (P <0 .0 0 1) ,并随时间推移呈下降趋势。结论 高浓度的TNF明显抑制促吞噬肽的产生 ,二者之间存在负反馈调节作用。首次提出了TNF -促吞噬肽轴的概念和二者临床应用的设想。证明了血清中促吞噬肽水平依赖于脾脏细胞的总量 ,保留一定数量的脾脏细胞对维持机体的免疫功能非常重要。
Objective To study the relationship between tuftsin and tumor necrosis factor α (TNF α). METHODS: Rat spleen and spleenless animal models were established. The concentration of TNF in serum was increased by intravenous injection of TNF under simulated pathological conditions. The level of phagocytic peptide in serum was detected by reversed-phase high performance liquid chromatography. Results The levels of pro-phagocytic peptides decreased in the splenectomized and splenectomized groups at 2 weeks postoperatively (P < 0.01), and the latter was. The levels of phagocytic peptides in the serum of d 3 and d 5 after injection of TNF in each group were significantly decreased (P < 0.01) and showed a decreasing trend over time. Conclusion High concentration of TNF significantly inhibits the production of phagocytic peptides, and there is a negative feedback regulation between them. For the first time, the concept of the TNF-phagosophin axis and its clinical applications were proposed. It is proved that the level of phagocytic peptide in serum depends on the total amount of spleen cells, and it is very important to maintain a certain amount of spleen cells to maintain the body’s immune function.