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目的:观察中药复方清热颗粒剂含药血清对低浓度抗生素环境下细菌耐药性的影响。方法:采用血清药理学方法,在中药复方清热颗粒剂含药血清和低浓度新霉素条件下传代培养大肠杆菌ATCC25922,以空白血清、低浓度新霉素、空白肉汤作对照,采用纸片扩散法(KB法),以抑菌环直径(mm)作为衡量标准,动态观察诱导菌株耐药性变化。结果:复方清热颗粒剂含药血清对大肠杆菌ATCC25922具有直接抑菌作用,末次灌胃给药60 min后含药血清抑菌环直径达10 mm;经低浓度新霉素诱导30代后,大肠杆菌ATCC25922不仅对新霉素产生耐药,而且导致丁胺卡那、氨苄西林失效,对庆大霉素、链霉素敏感性降低,产生多重耐药;新霉素+含药血清组菌株第40代菌株对新霉素产生耐药性,对丁胺卡那、氨苄西林、庆大霉素、链霉素始终保持敏感,其中氨苄西林的敏感性同空白肉汤组保持一致(P>0.05)。结论:复方清热颗粒剂具有延缓和控制细菌在低浓度抗生素诱导下产生耐药性以及多重耐药作用。
OBJECTIVE: To observe the effect of serum containing traditional Chinese herbal compound Qingre Granules on bacterial resistance to low concentrations of antibiotics. METHODS: E. coli ATCC25922 was subcultured using the serum pharmacology method under the conditions of traditional Chinese herbal compound Qingre Granule drug-containing serum and low concentration of neomycin. The blank serum, low concentration of neomycin, and blank broth were used as controls. Diffusion method (KB method), using the diameter of inhibition zone (mm) as a measure, dynamically observed changes in the resistance of induced strains. Results: The compound Qingre Granule drug-containing serum had direct bacteriostatic action against E. coli ATCC25922. The diameter of the antibacterial ring of the drug-containing serum reached 10 mm after 60 min of the last intragastric administration, and 30 days after the low concentration of neomycin induced the large intestine. Bacillus sp. ATCC25922 not only produces resistance to neomycin, but also results in the failure of amikacin and ampicillin, reduced sensitivity to gentamicin and streptomycin, and multidrug resistance; neomycin+ strain containing drug serum The 40 strains were resistant to neomycin and remained sensitive to amikacin, ampicillin, gentamycin, and streptomycin. The sensitivity of ampicillin was consistent with that of blank broth (P>0.05). ). Conclusion: Compound Qingre granules have the effect of delaying and controlling bacteria resistance and multidrug resistance induced by low concentrations of antibiotics.