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目的:探讨IL-1B-511基因多态性对幽门螺旋杆菌(H.pylori)感染后胃黏膜萎缩的影响。方法:(1)采用PC R-限制性长度片段多态性(restriction fragm ent length polym orphism,R FLP)分析法检测胃癌低发区广东省普通人群192例的基因型;(2)采用酶联免疫吸附法(E LISA)检测上述人群的H.pylori感染率、胃蛋白酶原I(pepsinogen I,PG I)、胃蛋白酶原II(pepsinogenII,PG II)和胃泌素(gastrin)的浓度。结果:H.pylori阳性者PG I/PG II显著低于H.pylori阴性者(P<0.01),H.pylori阳性的1L-1B-511T/T基因型者PG I/PG II比值显著低于C/C和C/T基因型者(均P<0.05)。血清胃泌素浓度与IL-1B-511的基因型没有明确的关系(P>0.05)。结论:在胃癌低发区,IL-1B-511基因型可能增加感染H.pylori后胃黏膜萎缩发生、发展的危险性。
Objective: To investigate the effect of IL-1B-511 gene polymorphism on gastric mucosal atrophy after H. pylori infection. Methods: (1) The genotypes of 192 cases of general population with low incidence of gastric cancer in Guangdong province were detected by PCR-RFLP (restriction fragment length polymorphism); (2) The H.pylori infection rate, the concentrations of pepsinogen I (PGI), pepsinogen II (PG II) and gastrin in the above-mentioned population were detected by E-ELISA. Results: The PG I / PG II of H.pylori positive patients was significantly lower than that of H.pylori negative patients (P <0.01). The PG I / PG II ratio of H.pylori positive 1L-1B-511T / T genotype was significantly lower than C / C and C / T genotypes (all P <0.05). Serum gastrin concentration and IL-1B-511 genotype no clear relationship (P> 0.05). Conclusion: IL-1B-511 genotype may increase the incidence and development of gastric mucosal atrophy after H. pylori infection in areas with low incidence of gastric cancer.