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目的:小鼠血浆中葛根素的含量测定及药代动力学研究。方法:采用高效液相色谱法,流动相为甲醇-水(23:77),流速1.0mL.min-1,检测波长250nm,柱温为室温。结果:葛根提取物口服后15min血液中即可检出葛根素,该药呈二室模型分布,其主要药动学参数为A=1121.105mg.L-1;B=237.5469m.L-1;α=0.6825h-1;β=0.0459h-1;Kα=1.3693h-1;t1/2α=1.0156h;t1/2β=15.0984h;K10=0.13h-1;K21=0.2305h-1;K12=0.362h-1;Cmax=456.6376mg.L-1;Tmax=1.2427h;AUC=5824.7144mg.h.mL-1。结论:葛根提取物口服后葛根素在小鼠体内吸收快、消除慢。
Objective: To determine the puerarin content and pharmacokinetics of mouse plasma. Methods: HPLC was used. The mobile phase was methanol-water (23:77), the flow rate was 1.0 mL.min-1, the detection wavelength was 250 nm, and the column temperature was room temperature. RESULTS: Puerarin could be detected in the blood of Pueraria lobata extract 15 min after oral administration. The drug was distributed in a two-compartment model. Its main pharmacokinetic parameters were A=1121.105 mg.L-1; B=237.5469 mL-1; 0.6825h-1; β=0.0459h-1; Kα=1.3693h-1; t1/2α=1.0156h; t1/2β=15.0984h; K10=0.13h-1; K21=0.2305h-1; K12=0.362 H-1; Cmax = 456.6376 mg.L-1; Tmax = 1.2427 h; AUC = 5824.7144 mg.h.mL-1. CONCLUSION: Puerarin is rapidly absorbed and eliminated slowly after oral administration of Pueraria lobata extract in mice.