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以抗、感大豆胞囊线虫4号生理小种的灰皮支黑豆和晋豆11为材料,人工接种胞囊线虫,在出苗后定期取样,测定抗、感品种接种与未接种处理根系苯丙氨酸解氨酶(PAL)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)活性以及根系内丙二醛(MDA)和超氧阴离子自由基(O.2-)含量的动态变化,以初步明确灰皮支黑豆抗大豆胞囊线虫4号生理小种的生化机制。结果表明,受大豆胞囊线虫4号生理小种侵染后,抗、感品种根系内PAL、SOD、POD、PPO酶活性均有增加,并且抗病品种根系酶活性增加相对较多,且持续时间长,而MDA和O.2-含量则在感病品种晋豆11中明显升高。在抗病品种根系中,接种前后MDA和O.2-含量变化不大。表明抗病品种比感病品种具有更强的防御能力和抗膜脂过氧化能力。
The resistant and susceptible Soybean cyst nematode No. 4 physiological races gray peel black beans and Jin Dou 11 as materials, artificial inoculation cyst nematode, regular sampling after emergence, determination of resistant and susceptible varieties of inoculated and non-inoculated roots (PAL), superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO) activity in roots and malondialdehyde (MDA) and superoxide anion radical O.2-) content of the dynamic changes in order to clarify the gray scale support Black soybean anti-Soybean Cyst 4 physiological race biochemical mechanism. The results showed that the activities of PAL, SOD, POD and PPO in roots and roots of resistant and susceptible cultivars increased with the inoculation of Soybean Cyst Nematode 4, and the enzyme activities of roots in resistant and susceptible cultivars increased relatively more A long time, while MDA and O.2-content was significantly increased in susceptible varieties Jin bean 11. In root system of resistant varieties, contents of MDA and O.2- did not change much before and after inoculation. The results showed that resistant varieties had stronger defense ability and anti-lipid peroxidation ability than susceptible varieties.