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以番茄品种“中蔬6号”为试材,采用生物信息学方法和实时荧光定量PCR技术,分析研究了番茄SlC3H64和SlC3H65锌指蛋白结构特点,启动子序列内所含胁迫响应元件类别,以及2个基因组织表达模式和非生物胁迫条件下表达量变化差异。结果表明:番茄SlC3H64和SlC3H65的蛋白预测结构差异较大;启动子序列分析发现,2个基因中分别含有11个和7个不同的胁迫响应元件,且二者除了TC-rich repeat元件相同外,其余元件均不同;SlC3H64和SlC3H65在“中蔬6号”根、茎和叶中都有表达,且都在叶中表达量最高,5种不同胁迫处理条件下,SlC3H64响应水杨酸(SA)和高温处理,而SlC3H65则仅响应甘露醇处理。
Using the bio-informatics method and real-time fluorescence quantitative PCR technique, we studied the structural characteristics of zinc finger proteins of tomato SlC3H64 and SlC3H65, and the types of stress response elements contained in the promoter sequence , As well as the difference in expression between two gene expression patterns and abiotic stress conditions. The results showed that the protein structure of SlC3H64 and SlC3H65 were different from each other. The promoter sequence analysis showed that 11 and 7 different stress response elements were contained in the two genes, respectively. Besides the same TC-rich repeat elements, The other components were different; SlC3H64 and SlC3H65 were expressed in roots, stems and leaves of “Zhongshu 6”, and all of them were expressed in the leaves at the highest level. Under the conditions of 5 different stresses, SlC3H64 responded to salicylic acid SA) and high temperature treatment, while SlC3H65 only responded to mannitol treatment.