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通过溶剂萃取与重结晶等方法从银莲花属植物多被银莲花Anemone raddeana Regel根茎中提取三萜皂苷银莲花素A,研究银莲花素A对非小细胞肺癌H460癌细胞的增殖、凋亡与细胞周期的影响及分子机制.结果表明:银莲花素A能显著地抑制H460癌细胞的增殖,且这种抑制效应呈明显的时间量效关系;能诱导H460癌细胞凋亡,并诱导其细胞周期阻滞于G2-M期;能抑制Akt激酶活性、下调Bcl-2蛋白表达以及激活PARP活性,从而导致其对H460癌细胞的增殖抑制作用.结果显示银莲花素A对非小细胞肺癌的预防与治疗可能具有潜在价值.
Extracting triterpenoid saponins Anemone A from the roots of Anemone raddeana Regel by solvent extraction and recrystallization, we studied the effects of Anemonin A on the proliferation, apoptosis and apoptosis of H460 non-small cell lung cancer cells Cell cycle and the molecular mechanism.The results showed that: Yin-Lian Hua A significantly inhibited the proliferation of H460 cancer cells, and the inhibitory effect showed a significant dose-time relationship; induced H460 cancer cell apoptosis and induced its cells Cycle arrest in the G2-M phase; can inhibit Akt kinase activity, down-regulate Bcl-2 protein expression and activation of PARP activity, resulting in its inhibition of proliferation of H460 cancer cells The results showed that the proliferation of non-small cell lung cancer Prevention and treatment may have potential value.