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杆状病毒表达载体系统为一种真核表达系统,在生物制药方面具有多种优势。测定杆状病毒滴度对病毒的扩增和重组蛋白的表达是非常必要的。本文系统地综述了蚀斑检测、测定β-半乳糖苷酶活性、终点稀释法、定量定时PCR、免疫染色检测法、使用微流控生物分析仪、测活细胞大小及用比色指示剂等8种方法测定杆状病毒滴度,各有其优缺点。
Baculovirus expression vector system is an eukaryotic expression system, which has many advantages in biopharmaceutical. Determining the titer of the baculovirus is very necessary for the amplification of the virus and the expression of the recombinant protein. This article systematically reviews the plaque detection, determination of β-galactosidase activity, the endpoint dilution method, quantitative PCR, immunostaining detection method, the use of microfluidic bioanalyzer, measuring cell size and using colorimetric indicators Eight methods for the determination of baculovirus titers have their own advantages and disadvantages.