论文部分内容阅读
目的 研究 L inomide对人大肠癌 SW1116裸鼠肠壁原位移植瘤的抑制及抗转移作用。方法 用肿瘤肠壁粘接原位接种新技术和脾切除的方法为肿瘤创造生长及转移的条件 ,经裸鼠体内筛选获得稳定模型 ,模型用于 L inom ide对 SW1116裸鼠肠壁原位移植瘤的抑制及抗转移作用的研究 ,设立丝裂霉素 (MMC)和不给药对照。结果 L inomide和 MMC对肠壁原位肿瘤的抑制率分别为 71.9%和 81.3% ,两种药物均抑制了肿瘤的转移 ,对照组的肿瘤肝转移率为 75 % (6 / 8)。结论 采用新的粘接接种技术和脾切除的方法 ,降低了受体的免疫力 ,不仅使模型稳定传代 ,而且还能产生自发转移 ,初步显示了此模型在研究转移机制及抗转移药物方面有一定的应用价值。
Objective To study the inhibitory and anti-metastatic effects of Lomomide on orthotopic transplanted tumors of human colorectal cancer SW1116 in nude mice. Methods Tumor intestinal wall adhesion in situ vaccination and splenectomy new methods of tumor growth and metastasis conditions, the stability of the model was screened by nude mice in vivo, the model for L inom ide SW1116 nude mice intestinal wall orthotopic transplantation Tumor inhibition and anti-metastasis of the study, the establishment of mitomycin (MMC) and no control. Results The inhibitory rates of Lomomide and MMC on the intestinal wall in situ tumors were 71.9% and 81.3%, respectively. Both drugs inhibited the metastasis of tumor, and the control group had a liver metastasis rate of 75% (6/8). Conclusions The new adhesive inoculation technique and splenectomy method can reduce the immunity of the recipient, which not only can make the model stable passage but also produce spontaneous metastasis, which initially shows that this model has many advantages in studying the metastatic mechanism and anti-metastatic drugs A certain value of the application.