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目的 :通过对同一宫颈癌患者放疗前、放疗 2周 10 Gy、4周 2 0 Gy连续宫颈肿物活检 ,Ki6 7、增殖细胞核抗原 (PCNA)、突变型 p5 3(mtp5 3)等增殖指标检测 ,探讨在后装分次放疗过程中加速再增殖出现时间。方法 :运用流式细胞仪行 Ki6 7抗原检测及细胞周期分析 S期比例 (SPF)、增殖指数 (PI)、DNA异倍体 (DEN) ,用免疫组化方法和计算机图像分析仪定量分析 PCNA、mtp5 3蛋白表达情况 ,观察各增殖指标随剂量增加时变化规律。结果 :Ki6 7、PCNA、mtp5 3抗原表达及 DEN随放疗剂量的增加、疗程的延长而明显增加 ,其中 Ki6 7、PCNA、mtp5 310Gy时与放疗前比较有统计学意义 ,与 2 0 Gy时比较差异无统计学意义。结论 :肿瘤细胞加速再增殖在宫颈癌后装分次放疗后 2周 10 Gy时已经出现 ,而 10 Gy与 2 0 Gy时各指标差异不明显 ,提示肿瘤细胞增殖速度稳定 ,为宫颈癌后装加速治疗时机的选择提供理论依据
OBJECTIVE: To detect the proliferation of cervical cancer patients before radiotherapy, 10 Gy 2 weeks after radiotherapy, 4 Gy 20 Gy cervical biopsy, Ki6 7, PCNA and mtp5 3 , To explore the time after accelerated sublimation radiotherapy in the process of proliferation and reproduction. Methods: Flow cytometry was used to detect Ki67 antigen and cell cycle analysis of S phase ratio (SPF), proliferation index (PI), DNA aneuploidy (DEN), immunohistochemistry and computerized image analyzer quantitative analysis of PCNA , Mtp5 3 protein expression, observe the proliferation of various indicators with increasing dose. Results: The expressions of Ki6 7, PCNA and mtp5 3 antigen and DEN increased with the increase of the dose of radiotherapy. The duration of treatment was significantly longer. The expression of Ki6 7, PCNA and mtp5 310Gy was significantly higher than that before radiotherapy. Compared with 20 Gy The difference was not statistically significant. CONCLUSION: Accelerated re-proliferation of tumor cells has occurred at 10 Gy two weeks after radiotherapy in patients with cervical cancer. However, there is no significant difference between 10 Gy and 20 Gy, suggesting that the proliferation rate of tumor cells is stable, Accelerate the choice of treatment timing to provide a theoretical basis