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用聚合兔IgG为固相抗原,抗人Ig的F(ab′)_2为酶结合物,建立了定量检测IgM、IgG、IgA类RF的ELISA方法。对135例正常人,86例类风湿性关节炎(RA)患者及162例其它疾病患者检测的结果提示,RA患者三类RF均明显升高;IgM、IgG、IgA类RF的检出率分别为80.2％,62.8％,和51.2％,三类RF均与血管炎的发生密切相关,与RA活动程度相关,以IgM-RF最显著。SLE等风湿病及其它内科疾病三类RF检出率为0～45％,某中干燥综合征(SS)、混合性结缔组织病(MCTD)IgG,IgA类RF检出率为40％左右。
Polymerized rabbit IgG as solid phase antigen, anti-human Ig F (ab ’) _2 as enzyme conjugate, established quantitative detection of IgM, IgG, IgA RF ELISA method. The results of 135 patients with normal subjects, 86 patients with rheumatoid arthritis (RA) and 162 patients with other diseases suggest that the three types of RF were significantly increased in RA patients. The detection rates of IgM, IgG and IgA RF were respectively Were 80.2%, 62.8%, and 51.2% respectively. All three types of RF were closely related to the occurrence of vasculitis, and were related to the degree of RA activity. The most significant was IgM-RF. SLE and rheumatism and other medical diseases three types of RF detection rate of 0 to 45%, a Sjogren’s syndrome (SS), mixed connective tissue disease (MCTD) IgG, IgA RF detection rate of about 40%.